Dry hopping rates in IPAs

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2021.11.30 18:15 stonk_frother Dry hopping rates in IPAs

I know, I know. IPAs are boring and we should all only make dark milds and stouts. But I like drinking IPAs, so sue me.
For all the other heathens out there who like brewing and drinking IPAs though, how heavily do you typically go on the dry hops?
In the past I used to go around 50-80 grams for a West Coast IPA, and around 100-120 grams for a NEIPA, assuming a regular 19 litre (5 gallons in Freedom Units) batch.
But when reading Scott Janish's recent blog post about The Locksmith, I was shocked to discover that he considers a 'normal' dry hop load to be 44lbs in a 10bbl batch - 4.4lbs/bbl, or around 16g/L. Now admittedly this is for a DIPA, but still, that would be around 300g in a typical 19L batch.
My rule of thumb is that a NEIPA usually gets 2/3 of the dry hops of a DIPA, and a WCIPA gets half the dry hops of a NEIPA. So if I apply this to Janish's hopping rates, it would be more like 100g for a WCIPA, 200g for a NEIPA, and 300g for a DIPA.
These numbers seem really high to me, and in the past I would've been worried about astringency. I'm starting to question though if that isn't another homebrewing myth.
So, IPA lovers, how heavily do you dry hop? Have you ever done 200, or 300 grams before? Did you have any issues with astringency or grassy flavours?
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2021.11.30 18:15 TomisMeMyselfandI Substrate profiling of mitochondrial caseinolytic protease P via a site‐specific photocrosslinking approach

Approaches for profiling protease substrates are critical for defining protease functions, but remain challenging tasks. Here we combine genetic code expansion, photocrosslinking and proteomics to identify substrates of the mitochondrial (mt) human caseinolytic protease P (hClpP). Site-specific incorporation of the diazirine-bearing amino acid DiazK into the inner proteolytic chamber of hClpP, followed by UV-irradiation of cells allows to covalently trap hClpP to substrate proteins and to substantiate hClpP’s major involvement in maintaining overall mt homeostasis. In addition to confirming many of the previously annotated hClpP substrates, our approach adds a diverse set of new proteins to the hClpP interactome. Importantly, our workflow allows to identify substrate dynamics upon external cues in an unbiased manner. Identification of unique hClpP-substrate proteins upon induction of mt oxidative stress, suggests that hClpP counteracts oxidative stress by processing of proteins that are involved in respiratory chain complex synthesis and maturation as well as in catabolic pathways.
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2021.11.30 18:15 TomisMeMyselfandI SnF2‐Catalyzed Formation of Polymerized Dioxolane as Solid Electrolyte and its Thermal Decomposition Behavior

Polymerized-dioxolane(P-DOL) is of potential as a solid-polymer-electrolyte(SPE) due to its high Li+-conductivity, good compatibility with Li-metal and desired preparation method of in-situ polymerization in cells. In this study, SnF2 was demonstrated not only to be an efficient catalyst for the polymerization of DOL at room temperature, but also an effective additive for improving interfacial wettability and suppressing dendrite through the reaction with Li-metal and the formation of LiF/LixSn based composite solid electrolyte interlayer(SEI). Using the SnF2 polymerized P-DOL containing 1M LiTFSI as SPE(P-DOL-SPE), obviously denser Li-deposition was obtained, and the all-solid-state(ASS) Li/LiFePO4 cell delivered stable cycling over 350 cycles at 45oC. At the same time, the irreversible decomposition of P-DOL-SPE into formaldehyde and small molecule epoxides are observed at 110oC, which is even initiated at lower temperature of 40oC under vacuum. This thermal decomposition of P-DOL-SPE in pouch cell causes huge volume swell, and therefore putting a strict limitation on the operating temperature window for the P-DOL based electrolytes.
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2021.11.30 18:15 TomisMeMyselfandI Rational Design of Axially Chiral Styrene‐Based Organocatalysts and Their Application in Catalytic Asymmetric (2+4) Cyclizations

A new class of axially chiral styrene-based thiourea-tertiary amine catalysts, which have unique characteristics such as an efficient synthetic route, multiple chiral elements and multiple activating groups, has been rationally designed. These new chiral catalysts have proven to be efficient organocatalysts, enabling the chemo-, diastereo- and enantioselective (2+4) cyclization of 2-benzothiazolimines with homophthalic anhydrides in good yields (up to 96%) with excellent stereoselectivities (all >95:5 dr, up to 98% ee). More importantly, theoretical calculations elucidated the important role of axially chiral styrene moiety in controlling both the reactivity and the enantioselectivity. This work not only represents the first design of styrene-based chiral thiourea-tertiary amine catalysts and the first catalytic asymmetric (2+4) cyclization of 2-benzothiazolimines, but also gives an in-depth understanding of axially chiral styrene-based organocatalysts.
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